J Agric Food Chem. Appl Microbiol Biotechnol. The principles of HRMAS have been extensively discussed previously [6–10]. R) around an axis inclined of an angle of 54.7° (the so-called “magic angle”) to the static magnetic field (B The ellipsoid regions circumscribe the dense cluster of F (red) or G (green) signals. NMR Biomed. PubMed Central  The authors reported that snow vole activity was capable to significantly affect the humification process and SOM cycling, with effects on the hormone-like bioactivity of humic fractions. The wide variety of materials (including in vivo micro- and macroorganisms) successfully studied by HRMAS suggests that it is the best choice when standard solution- and solid-state NMR techniques are not applicable or unsatisfying. Moreover, edited (namely “filtered”) pulse sequences, such as T2- and diffusion-based sequences, can be used in HRMAS systems [10–12]. Biomass production was larger in treatment (i), whereas the specific ethanol production rate upon glucose addition was similar in both treatments, thus implying that oxygen availability affected the respiration, but not the yeast fermentation. Eur J Soil Sci. It also can help to understand the treatment of that certain diseases. 2010;6:121–5. Anal Chem. Available from: http://dx.doi.org/10.5772/48340). The metabolomic profile of the plant leaves revealed that the pesticide affected the concentration of a number of lettuce metabolites, thus indicating a large oxidative stress and a Krebs cycle up-regulation at a late stage of plant growth. HR-MAS NMR spectroscopy in material science. Adv Agron. The SNIF-NMR analytical method was developed to detect the (over) sugaring of wine and enrichment of grape musts, and is mainly used to check the authenticity of foodstuffs (such as wines, spirits, fruit juice, honey, sugar and vinegar) and to control the naturality of some aromatic molecules (such as vanillin, benzaldehyde, raspberry ketone and anethole). Combourieu B, Inacio J, Delort AM, Forano C. Differentiation of mobile and immobile pesticides on anionic clays by 1H HR MAS NMR spectroscopy. London: Royal Society of Chemistry; 2013. p. 164–70. https://doi.org/10.1186/s40538-017-0093-9, DOI: https://doi.org/10.1186/s40538-017-0093-9. Part of 2014;159:236–43. Insights into the structure of cutin and cutan from Agave americana leaf cuticle using HRMAS NMR spectroscopy. Intech Open; 2012. p. 279–306. Int J Mol Med. 1), loaded into the HRMAS probe, and spun at a relatively moderate rate (ν As for other biological materials HRMAS has been usefully applied to rapidly monitor the plant stress in response to both abiotic and biotic factors, such as drought, salinity, nutrients deficiency, or pathogens influence. NMR spectroscopy. Feng X, Simpson AJ, Simpson MJ. © 2021 BioMed Central Ltd unless otherwise stated. Food Chem. Lam et al. 2010;23:1–10. This allows HRMAS spectroscopy to provide information on both polar and non-polar components of biological tissues or agrofood products without preliminary separations though extractions. Would you like email updates of new search results? In addition, the authors studied the survival extent of these amphipods as a function of oxygen availability, temperature, and organism orientation into the NMR rotor. Liaghati Mobarhan Y, FortierMcGill B, Soong R, Maas WE, Fey M, Monette M, et al. statement and [Figure reprinted with permission from Mazzei et al. A feasibility study. Alam TA, Jenkins JE. Advances of high-resolution NMR techniques in the structural and metabolic analysis of plant biochemistry. The authors were able to detect through HRMAS the presence of withaferin A and withanone molecules, which are secondary metabolites produced by this plant and important natural medicinal compounds [42]. Later, the same research group used a similar 1H- and 13C-HRMAS approach to characterize two Italian cultivars of garlic (Allium sativum L.) [62]. For example, seeds, peel, flesh, and puree of tomatoes were studied by HRMAS to produce a metabolomic profiling of Spanish Almeria tomatoes and identify the molecular markers correlated to ripening process [59]. COVID-19 is an emerging, rapidly evolving situation. Y1 - 1990 Ritota M, Casciani L, Han BZ, Cozzolino S, Leita L, Sequi P, et al. Valentini M, Ritota M, Cafiero C, Cozzolino S, Leita L, Sequi P. The HRMAS–NMR tool in foodstuff characterization. Phytochemistry. Food Chem. Food Res Int. Org Geochem. Recently, HRMAS revealed the molecular composition of crocus sativus petals and detected several bioactive compounds such as kinsenoside, goodyeroside A, and 3-hydroxy-γ-butyrolactone [46]. Magn Reson Chem. 2013;141:2841–7. Annu Rev Biochem. NMR-based metabolomics for organic farming traceability of early potatoes. 2011;44:3212–21. A detailed metabolic profiling of different tissues (flavedo, albedo, seeds, and pulp) of both lemon and citrus fruits was conducted through HRMAS as well as they characterized several compounds associated with the development of mold in the citron flavedo [57]. II: HR MAS. Increased levels of triglycerides observed in the mutant were interpreted as indicative of insulin resistance with implications for mitochondrial disfunction. Delgado-Goni T, Campo S, Martın-Sitjar J, Cabanas ME, San Segundo B, Arus C. Assessment of a 1H high-resolution magic angle spinning NMR spectroscopy procedure for free sugars quantification in intact plant tissue. 2014;154:291–8. The authors observed a significant enhancement of seedlings germination accompanied by a change in leaf metabolome that was treatment-specific, thereby inferring that the method may help in developing new biofertilizers based on fungi extracts [14]. 2016;214:539–48. 2016;7:4856–66. Assessing the fate and transformation of plant residues in the terrestrial environment using HR-MAS NMR spectroscopy.  |  The figure shows the disposition of both RF solenoid and gradient coils as well as indicates the inclination of rotation axis at the magic angle (θ = 54.7°) in respect of static magnetic field (B Environ Sci Technol. Vermathen M, Marzorati M, Baumgartner D, Good C, Vermathen P. Investigation of different apple cultivars by high resolution magic angle spinning NMR. 2009;195:77–88. Unraveling the long-term stabilization mechanisms of organic materials in soils by physical fractionation and NMR spectroscopy. Silva LMA, Alves Filho EG, Choze R, Lião LM, Alcantara GB. Messia MC, Sobolev AP, Gómez-Caravaca AM, Lamanna R, D’Amico I, Caboni MF, Marconi E, et al. The relatively recent and advanced high-resolution magic angle spinning (HRMAS) NMR technique enables the direct application of NMR spectroscopy to semi-solid and gel-like samples. Courtier-Murias D, Farooq H, Masoom H, Botana A, Soong R, Longstaffe JG, et al. The emphasis is the practicality of the solution and “getting the job done”. Later, the same authors adopted a similar analytical approach to identify the structure of cutan and a cutin/cutan mixture from the Agave americana leaf cuticle [39]. The principal applications of high-resolution solid-state NMR spectroscopy, in the field of food science, are reviewed, after a short general introduction, mainly focusing on the potential of these investigations, which are, today, routine tools for resolving technological problems. J Agric Food Chem. Briefly, each semi-solid sample is reduced to small pieces, fit into a HRMAS rotor, and added with a small amount of deuterated solvent to ensure both the molecular spin mobility and the deuterium lock. Mol Biosyst. HHS 2003;64:1163–70. PubMed  Winning H, Viereck N, Wollenweber B, Larsen FH, Jacobsen S, Søndergaard I, et al. A new metabonomic strategy for analysing the growth process of the poplar tree. Mucci A, Parenti F, Righi V, Schenetti L. Citron and lemon under the lens of HR-MAS NMR spectroscopy. J Apic Res. One important feature of NMR 2013;52(3):141–8. Later, the same research group used HRMAS to differentiate three varieties of tomatoes and the basis of their composition [60]. In particular, the abiotic stress favored the accumulation of free amino acids and saccharides. Metabolomic analysis at an early stage of regeneration. Epub 2014 Apr 29. A 3D HRMAS spectroscopy was applied to examine tomato cutin and then compared to solution-state spectra of soil extracts from the Ah horizon under an oak forest [18]. Piccolo A, Campanella L, Petronio BM. 2011;49:S121–5. Centro Interdipartimentale per la Risonanza Magnetica Nucleare per l’Ambiente, l’Agro-Alimentare ed i Nuovi Materiali (CERMANU), Università di Napoli Federico II, Via Università 100, 80055, Portici, Italy, You can also search for this author in Food Chem.  |  The presumed beneficial role exerted by humic acids in plant regeneration was verified by HRMAS on root and shoot calli explanted by pear and quince plants, after treatments with different concentrations of peat humic acids [45]. Nat Protoc. NUCLEAR MAGNETIC RESONANCE SECTROSCOPY 2. iNTRODUCTION Powerful analytical technique used to characterize organic molecules by identifying carbon-hydrogen frameworks within molecules. Bon D, Gilard V, Massou S, Pérès G, Malet-Martino M, Martino R, et al. The authors created epitope maps (Fig. The application of T2-based (namely CPMG or spin-echo) NMR filters to reduce signals heterogeneity in 1H spectra of humic substances allowed to develop cluster analyses to differentiate humic matter as a function of soil treatments [25]. The supramolecular structure of humic substances. Finally, Capitani et al. Mazzei P, Piccolo A. Interactions between natural organic matter and organic pollutants as revealed by NMR spectroscopy. NMR Spectrometer and Data Collection: A brief description of a NMR spectrometer and its working with attention to locking, shimming, tuning, and parameter optimizations. In fact, ripening produced a decrease of citric acid accompanied by an increase of lactic acid, gamma aminobutyric acid, phenylalanine, and niacin. Protons which have 80–100% binding are highlighted by red circles, protons with 65–79% with blue, and 64% and lower with green. HR-MAS NMR metabolomics of ‘Swingle’ citrumelo rootstock genetically modified to overproduce proline. Copyright 2016 by J. Agric. 2003;37:337–42. Snow vole (Chionomys nivalis Martins) affects the redistribution of soil organic matter and hormone-like activity in the alpine ecosystem: ecological implications. CAS  It combines the advantages of both solid- and liquid-state NMR by allowing to concomitantly measure intact and non-manipulated samples. : https: //doi.org/10.1186/s40538-017-0093-9 and histopathological alterations of Jatropha mosaic begomovirus [ ]... Sequi P. the identification of carbohydrates in beer has been repeatedly applied to in vivo 31P and HR-MAS. 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