Browse selective and differential media types in broth or agar for Escherichia coli (E. coli) and coliforms. A complex medium in which the exact amounts of components and Examples include sheep blood agar and chocolate (heated blood) agar. A medium containing many growth factors. Undefined medium. While differential media is used in order to differentiate between closely related organisms.Examples are: McConkey, Blood, and EMB Agars. Three of the more common selective and differential media are described below and will be used in the laboratory exercise. What is the Difference Between Selective and Differential Media      – Comparison of Key Differences, Key Terms: Antibiotic Resistance, Biochemical Characteristics, Differential Media, Indicators, Marker, Microorganisms, Selective Media. Selective media are used to isolate a particular type of microorganism by giving a specific condition for the growth of that particular microorganism. Selective Media: Selective media do not use indicators. Selective and differential media are two types of media used to isolate and identify microorganisms. The gene that gives the ability to grow in the selective medium is known as the marker. Differential media, unlike selective media, does not kill organisms. Functionally, selective media permit certain types of organisms to grow while inhibiting the Terrific Broth (TB) is used with glycerol in cultivating recombinant strains of Escherichia coli. Both selective and differential media are used to isolate and identify microorganisms. What is the Difference Between Mushrooms and... What is the Difference Between Black Mold and Mildew, What is the Difference Between Zoospore and Zygospore. MAC is a Selective Medium because it encourages some bacteria to grow while inhibiting others. (OpenStax CNX, 2018) Selective media are formulated with inhibitors such as antibiotics or high NaCl concentration. Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. There are many types of media used in the studies of microbes. In addition to MacConkey’s agar only growing Gram-negative bacteria (the selective aspect of the medium), MAC has special additives that cause lactose fermenting bacteria (microbes that can For more information contact us at info@libretexts.org or check out our status page at https://status.libretexts.org. Pankaj, presumably you mean differential media as used in biological and medical tests? What is Differential Media      – Definition, Role, Examples 3. Selective media allows the selection of a group of organisms but not others.Examples are: McConkey Agar, Blood Agar, Mannitol Salt Agar, and EMB. MacConkey agar for Gram-negative bacteria. Differential Media: Differential media use indicators. Some Examples of Selective and Differential Media: Mannitol Salt Agar (MSA) Mannitol Salt Agar (MSA) is a selective medium for the isolation of pathogenic and non-pathogenic Staphylococci. Selective growth media are also used in cell culture to ensure the survival or proliferation of cells with certain properties, such as antibiotic resistance or the ability to synthesize a certain metabolite. The most common growth media for microorganisms are nutrient broths (liquid nutrient medium) or lysogeny broth medium. For example, mannitol salt agar contains a high concentration of sodium chloride that inhibits the growth of most organisms but permits staphylococci to grow. Examples of differential media include: The LibreTexts libraries are Powered by MindTouch® and are supported by the Department of Education Open Textbook Pilot Project, the UC Davis Office of the Provost, the UC Davis Library, the California State University Affordable Learning Solutions Program, and Merlot. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. MacConkey agar is an example of a medium that is both differential and selective. Watch the recordings here on Youtube! MANNITOL SALT AGAR (MSA) Mannitol salt agar is a selective medium used for the isolation of pathogenic staphylococci. MacConkey Agar (or MAC) is a Selective & Differential Medium. 2. Understand how the different bacteria grow on these media. Two types of media with similar implying names but very different functions, referred to as selective and differential media, are defined as follows. Figure 2: Selective (right) and Differential (left) Media, Contains bovine heart blood that becomes transparent in the presence of hemolytic, Differential for lactose and sucrose fermentation. ENRICHED MEDIA. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. A selective media only permits the development of particular organisms, while a differential media discerns one species of the organism from the other growing on the similar substrate. Home » Science » Biology » Microbiology » Difference Between Selective and Differential Media. Selective medium. The main difference between selective and differential media is their role in the identification of microorganisms. Differential media is also known as indicator media. Selective Media: Selective media are used to isolate a particular strain of microorganisms. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. For example: Selective media: YM agar selects for microbes that grow in low pH conditions such as yeasts and molds. SELECTIVE MEDIA. Selective, and Differential Media Rich (or enriched) medium. An example of differential/selective media used in this experiment was Mannitol Salt agar used to isolated the bacterium Staphylococcus aureus and Staphylococcus epidermidis. Some examples of selective media are shown in the table below. Eosin methylene blue (EMB) that contains methylene blue – toxic to Gram-positive bacteria, allowing only the growth of Gram negative bacteria. MacConkey (MCK), which is differential for lactose fermentationmannitol salt agar (MSA), which is differential for mannitol fermentation. Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called selective media. While these are just a few examples of how types of media can help microbiologists distinguish between microbes, there are many other types of selective and differential media. Selective and differential media are two types of growth media used to grow microorganisms. 1. Moreover, media can be prepared that can distinguish between different kinds of microbes, such as Gram-positive and Gram-negative bacteria. “Culturing Media.” Boundless Microbiology, Available here. Selective growth media for eukaryotic cells commonly contain neomycin to select cells that have been successfully transfected with a plasmid carrying the neomycin resistance gene as a marker. These formulations may include antibiotics which inhibit the growth of gram positive bacteria or gram negative bacteria. Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. Specialized media can be selective; formulated to grow only certain microbes while inhibiting ​the growth of others. Kinsley’s medium contains polymyxin-B that enables isolation of Bacillus anthracis. Mannitol Salt medium (MSA) will only grow halophilic (salt-loving) bacteria, such as Staphylococcus & Micrococcus. What is Selective Media       – Definition, Role, Examples 2. Liquid media are often mixed with agar and poured via a sterile media dispenser into Petri dishes to solidify. In such cases, the gene is termed a marker. Differential media refer to a type of growth media that allows the differentiation of closely-related microorganisms. Selective media inhibit the growth of certain bacteria and allow for the growth of others.This permits the isolation of desired bacteria in pure subcultures.An example of a selective media is Coulmbia CNA Agar which contains two antibiotics: Colistin and Nalidixic acid to inhibit Gram negative bacteria, leaving only the GRAM POSITIVES to grow. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. Eukaryotic cells can also be grown in selective media. Potassium tellurite medium used to recover C. diphtheriae contains 0.04% potassium tellurite. Potassium tellurite medium used to recover C.diphtheriae contains 0.04% potassium tellurite. Mannitol Salt Agar and Salt Milk Agar used to recover S.aureus contains 10% NaCl. Mannitol salt agar (MSA) which is selective for Gram-positive bacteria and differential for mannitol. So, media could be both selective as well as differential. These agar plates provide a solid medium on which microbes may be cultured. Mannitol Salt Agar and Salt Milk Agar used to recover S. aureus contains 10% NaCl. Differential (contains mannitol and indicator phenol red): pathogenic Staphylococci ferments mannitol, producing an acid and turning media yellow. The media are enriched usually by adding blood, serum or egg. Example of selective media added with chemicals: The presence of bile salts, as well as crystal violet, within the media prevent gram … Selective Media: Selective media refer to a type of growth media that allows the growth of selected microorganisms in the medium. What is the Difference Between Streak Plate and Pour... What is the Difference Between Asteroid and Meteorite, What is the Difference Between Seltzer and Club Soda, What is the Difference Between Soda Water and Sparkling Water, What is the Difference Between Corduroy and Velvet, What is the Difference Between Confidence and Cocky, What is the Difference Between Silk and Satin. Unless otherwise noted, LibreTexts content is licensed by CC BY-NC-SA 3.0. Our microbiological media aid in the isolation, differentiation, detection, and enumeration of pathogens in food, water, clinical material, and more. A microorganism that grows on a selective agar medium is shown in figure 1. Hektoen enteric agar (HE) which is selective for Gram-negative bacteria. Selective media contain ingredients that inhibit the growth of some organisms but allow others to grow. Selective media can include dyes and other chemicals such as antibiotic types, specifically neomycin, that prevent the growth of some types of cells. This type of media is used for the detection of microorganisms and by molecular biologists to detect recombinant strains of bacteria. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. 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